The viability of sperm in lateral oviducts and spermathecae of instrumentally inseminated and naturally mated honey bee (Apis mellifera L.) queens

publication date: Jul 11, 2011
Send a summary of this page to someone via email.
Journal of Apicultural Research
Vol. 50 (3) pp. 190 - 194
DOI
10.3896/IBRA.1.50.3.02
Date
July 2011
Article Title

The viability of sperm in lateral oviducts and spermathecae of instrumentally inseminated and naturally mated honey bee (Apis mellifera L.) queens

Author(s)
H Vasfi Gençer and Yasin Kahya
Abstract

Instrumentally inseminated queens (IIQ) were compared with naturally mated ones (NMQ) to detect the effect of the instrumental insemination procedure on sperm viability in the reproductive organs. The viability of sperm in the reproductive organs of IIQ and NMQ was determined at 4 h and 24 h after they were inseminated or they mated. No significant difference was found between IIQ (88.6%) and NMQ (87.6%) in viability of sperm in the lateral oviducts at 4 h. There was also no difference between IIQ (98.3%) and NMQ (97.8%) in viability of sperm in spermathecae at 4 h. The mean viability of sperm in spermathecae of IIQ (97.5%) did not differ from the mean viability of sperm in spermathecae of NMQ (96.8%) after 24 h. These results showed that the insemination procedure had no negative effect on viability of sperm in reproductive organs of queens. The difference in sperm viability between lateral oviducts (88.1%) and spermathecae (98.1%) of queens at 4 h was found to be statistically significant (P < 0.001). Nevertheless, no significant difference was found between viability of sperm in spermatheca at 4 h (98.1%) and 24 h (97.2%). The possible reasons for the differences in viability of sperm in reproductive organs are discussed. 

Keywords

honey bee, queen, drone, natural mating, instrumental insemination, sperm viability, sperm migration, spermatheca, lateral oviduct.

Full text
pdf
Free to Subscribers Download button     Buy Now Download Button