The detection of acute paralysis virus in Varroa jacobsoni by the use of a simple indirect ELISA

publication date: Jul 28, 2011
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Journal of Apicultural Research
Vol. 25 (2) pp. 100-105
Date
June 1986
Article Title

The detection of acute paralysis virus in Varroa jacobsoni by the use of a simple indirect ELISA

Author(s)

M. F. Allen I, B. V. Balli, R. F. White And J. F. Antoniw

Abstract

A simple indirect enzyme-linked immunosorbent assay (ELISA) was used to detect and quantify acute paralysis virus (APV) in individual female Varroa jacobsoni collected from naturally infested honeybee (Apis mellifera) colonies in the Netherlands and Germany. The assay was very specific and sensitive, detecting the virus in concentrations as low as 3 ng/ml. Mites collected from both adult honeybees and sealed brood cells contained APV but virus concentrations varied widely between.

Keywords

Bee disease, Acute paralysis virus, APV, varroa jacobsoni, enzyme-linked immunosorbent assay, ELISA

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