Standard methods for American foulbrood research

publication date: Jan 29, 2013
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Journal of Apicultural Research
Vol. 52 (1)
DOI
10.3896/IBRA.1.52.1.11
Date
January 2013
Article Title

Standard methods for American foulbrood research

Author(s)

Dirk C de Graaf, Adriana M Alippi, Karina Antúnez, Katherine A Aronstein, Giles Budge, Dieter De Koker, Lina De Smet, Douglas W Dingman, Jay D Evans, Leonard J Foster, Anne Fünfhaus, Eva Garcia-Gonzalez, Aleš Gregorc, Hannelie Human, K Daniel Murray, Bach Kim Nguyen, Lena Poppinga, Marla Spivak, Dennis VanEngelsdorp, Selwyn Wilkins and Elke Genersch.

Abstract

American foulbrood is one of the most devastating diseases of the honey bee. It is caused by the spore-forming, Gram-positive rod-shaped bacterium Paenibacillus larvae. The recent updated genome assembly and annotation for this pathogen now permits in-depth molecular studies. In this paper, selected techniques and protocols for American foulbrood research are provided, mostly in a recipe-like format that permits easy implementation in the laboratory. Topics covered include: working with Paenibacillus larvae, basic microbiological techniques, experimental infection, and “’omics” and other sophisticated techniques. Further, this chapter covers other technical information including biosafety measures to guarantee the safe handling of this pathogen.

Keywords

honey bee, American foulbrood, Paenibacillus larvae, brood, disease, pathogen, technique, COLOSS, BEEBOOK

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