Source of the honey protein responsible for apple juice clarification
C. Y. Lee, N. L. Smith, R. W. Kime and R. A. Morse
Samples of honey from various floral sources and 'honey' from sucrose-fed bees (Apis mellifera) were compared for their activity in clarifying apple juice. Activity was directly related to honey protein content. Proteins were separated by column chromatography and tested for their ability to clarify apple juice. Regardless of their origin, all honey samples, including sucrose 'honey', contained the same two major protein components, as indicated by gel electrophoresis. One of these showed clarifying activity in apple juice. It was concluded that the honey protein responsible for clarifying apple juice originates in honeybees.