Sodium dodecyl sulphatepolyacrylamide profiles and western blots of Bacillus larvae
M A Z Hornitzkv; S P Djordjevic
American foulbrood (AFB), caused by Bacillus larvae, is a significant disease of honey bees. To date, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SOS-PAGE) and immunoblotting have not been reported as a means of determining the relatedness of different strains of B. larvae. These techniques are useful aids in the identification of species and subtypes of a range of bacteria and mycoplasma (Moore et al., 1980; Jones et al., 1976) and for showing similarities and variability among such species (Brown et al., 1991; Thirkell et al.,1990).
Bacillus larvae, strains, SOS-PAGE, immunoblotting, bacterial proteins, Australia