The release of Nosema apis spores from the epithelium of the honeybee midgut
T. P. Liu
When spores of Nosema apis matured, the epithelial cells became distended and were shed into the lumen of the midgut. Non-infected cells were also shed into the lumen. The infected cells contained lysosomes and the cytoplasm was extensively lysed. The non infected cells contained lysosomes, zymogen granules, multivesicular bodies, endoplasmic reticulum and mitochondria. Newly shed cells were intact, with microvilli attached. In the lumen, the microvilli, the plasma membrane and the cytoplasm were lysed. Mature spores of N. apis were then released into the midgut lumen. The mechanism of releasing spores from the lysed epithelial cells is discussed.