Effect of extraction solvent on antiradical activity of the obtained propolis extracts

publication date: Feb 26, 2014
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Journal of Apicultural Research
Vol. 53 (1) pp. 91-100
February 2014
Article Title

Effect of extraction solvent on antiradical activity of the obtained propolis extracts


Eduardo Morgado Schmidt, Cerize da Silva Santos, Daniele Stock, Daiane Finger, Wilhelm Josef Baader, Isis Kaminski Caetano, Sueli Pércio Quináia, Alexandra Christine Helena Frankland Sawaya, Marcos Nogueira Eberlin and Yohandra Reyes Torres


Total phenolic, flavonoid and mineral contents and antiradical activity are usually used as a measure of propolis extract quality. The purpose of this study was to compare the quality of propolis extracts obtained with different solvents such as water, ethanolic solutions (30, 70, 95%, v/v in water) and edible vegetable oils (canola and soybean). LC-MS was used to identify constituents of the extracts. Antiradical activity was assayed using chemiluminescence from the luminol/hemin/H2O2/CTAB system and the DPPH test. Both antiradical assays were strongly correlated (r = -0.97). Principal Component Analysis indicated positive correlations for phenolics, flavonoid levels and antiradical activity showing that all phenolic compounds are important for the scavenging activity of Brazilian propolis. The extract obtained with ethanol 30% v/v had the highest antiradical activity mainly due to its high content of dicaffeoylquinic acids.


propolis, extract quality, phenolics, antiradical activity, chemiluminescence reactions, DPPH, principal components analysis

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