The effects of low doses of gamma irradiation on the ultrastructure of Nosema apis in vitro
T. P. Liu, T. A. Batchelor, R. J. Munn, J. M. Marston And C. L. Judson
Infected midgets of honeybees in the tissue culture medium received low dose gamma irradiation. After irradiation dramatic ultrastructural changes took place in various developmental stages of Nosema apis. In the irradiated sporoblasts and sporont stages laminated endoplasmic reticulum was absent instead there were numerous vacuoles in the cytoplasm. There were fewer nuclear pores in the nuclear envelope of irradiated. organisms than in the untreated group. The diameter of the nuclear pore was larger in the irradiated group. The polar filament was not developed in the irradiated sporoblast. There were very few mature spores developed in the midgut 48 h after irradiation.