Success rates for instrumental insemination of carbon dioxide and nitrogen anaesthetised honey bee (Apis mellifera) queens

publication date: Feb 1, 2012
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Journal of Apicultural Research
Vol. 51 (1) pp. 74 - 77
DOI
10.3896/IBRA.1.51.1.09
Date
February 2012
Article Title

Success rates for instrumental insemination of carbon dioxide and nitrogen anaesthetised honey bee (Apis mellifera) queens

Author(s)

Bożena Chuda-Mickiewicz, Krystyna Czekońska, Jerzy Samborski and Piotr Rostecki

Abstract

This study assessed the usefulness of CO2 / N2 mixtures for anaesthetising queen bees (Apis mellifera) in instrumental insemination. Of 98 instrumentally inseminated queens, 31 were anaesthetised with 40% CO2 and 60% N2, 32 with 60% CO2 and 40% N2, and 33 with 100% CO2. The gas composition did not affect the proportion of queens starting oviposition, which were: 85.3% for 40% CO2 and 60% N2; 87.9% for 60% CO2 and 40% N2; and 83.9% for 100% CO2. On average, queens started oviposition significantly earlier in the treatments using 60% CO2 and 40% N2 (at 7.2 ± 4.0 days after insemination) and 100% CO2 (at 8.1 ± 4.2 days) than those treated with 40% CO2 and 60% N2 (at 10.3 ± 4.1 days).

 

Keywords

Apis mellifera, queen bee, carbon dioxide, nitrogen, instrumental insemination

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