Fingerprinting and assessment of genetic variability of Varroa destructor in Egypt

publication date: Jun 23, 2010
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Journal of Apicultural Research
Vol. 49 (3) pp. 251-256
DOI
Date
July 2010
Article Title

Fingerprinting and assessment of genetic variability of Varroa destructor in Egypt

Author(s)

Nabil. S. Awad, Sally F. M. Allam, Margueriet A. Rizk, Morad. F. Hassan and Ayman.Y. Zaki

Abstract

This study describes the use of eleven Random Amplified Polymorphic DNA (RAPD) primers to determine the genetic variability of Varroa destructor mites in Egypt. Mites from ten different Egyptian localities were included in this study. The results indicated that 133 bands were amplified, out of a total of 923. Of these amplified bands, 95 were polymorphic (71.4%) and 38 were monomorphic (28.6%). In addition, 37 specific positive RAPD markers for V. destructor in Egypt were identified. This is the first molecular genetic study carried out with V. destructor in Egypt. The markers obtained in the present study might have potential applications mite control for the beekeeping industry in Egypt. Further detailed investigations at DNA sequence level are needed to obtain complete and accurate identification of V. destructor in Egypt.

Keywords

Egypt, genetic variability, Varroa destructor, mites, RAPD-PCR, fingerprint, DNA

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