Enzyme levels used to monitor pesticide resistance in Varroa jacobsoni

publication date: Dec 8, 2010
Send a summary of this page to someone via email.
Journal of Apicultural Research
Vol. 30 (1) pp. 17-20
Date
March 1991
Article Title

Enzyme levels used to monitor pesticide resistance in Varroa jacobsoni

Author(s)

Uri Gerson; Rita Mozes-Koch; Ephraim Cohen

Abstract

The contingency of pesticide resistance in the mite Varroa jacobsoni Oudemans prompted this preliminary study, in which methodology for assaying levels of two detoxification systems, namely esterases and glutathione Stransferase (GST), was developed. The total esterolytic activity detected in post- mitochondrial fractions of a homogenized 200-mg sample of V. jacobsoni from 10 colonies was 0.0068 (00) min-1~g-1 protein. GST activity, expressed in ~mol conjugate min-1~g-1 protein was 0.260±0.21 in the post-mitochondrial supernatant and 0.646±0.48 in the cytosol. These results pertain only to mites obtained from a single experimental apiary of A. mellifera Iigustica in Israel. The methodology and preliminary results obtained may be used for subsequent local or regional monitoring of enzyme levels associated with pesticide resistance in V. jacobsoni.

Keywords

Varroa jacobsoni, pesticide resistance, detoxification systems, esterases, glutathione S-transferase, honey bees, Apis mellifera.

Full text
pdf
Free to Subcribers button     Buy Now for £5 button