Effect of above freezing temperatures on temporary storage of honeybee spermatozoa

publication date: Jun 10, 2011
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Journal of Apicultural Research
Vol. 26 (1) pp. 53-55
Date
March 1987
Article Title

Effect of above freezing temperatures on temporary storage of honeybee spermatozoa

Author(s)

John R. Harbo And Jon L. Williams

Abstract

Honeybee (Apis mellifera) semen was diluted 1 : 1 with saline and stored at 5, 10, 15, 20, 25 or 35°C for 2 days, or was not stored (control). Each of 111 queens was inseminated with 4 ml of the diluted semen. The effects of storage temperatures were evaluated by counting the number of spermatozoa that entered the spermatheca. Mean counts were ≤3.1 x 105 when spermatozoa were stored ≤ 10°C; storage at 20°C and 25°C resulted in the highest counts (mean = 1.4 x 106). The response in millions of spermatozoa in the spermatheca (Y) to storage temperature between 10 and 25°C (X) was Y = -2.74 + 0.397X - 0.00935X2 (r = 0.78, n= 34).

Keywords

bee breeding, storing spermatoza, temperature

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