Distribution of protein fractions in the blood of worker brood of Apis mellifera

publication date: Aug 21, 2011
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Journal of Apicultural Research
Vol. 24 (2) pp. 80-85
Date
June 1985
Article Title
Distribution of protein fractions in the blood of worker brood of Apis mellifera
Author(s)
Z. F. Glinski and J. Jarosz
Abstract
Samples of blood were taken from larvae which had straightened in their cells (10-11 days old), and from young pupae. Polyacrylamide gel electrophoresis of the samples after they had been strongly dissociated using a buffer, made possible the determination of detailed distributional and quantitative characteristics of the blood proteins, including the determination of molecular weights of particular protein fractions or their subunits. Densitometer scans revealed 24 well-defined fractions in larval blood and 27 in pupal blood. It was shown that after dissociation almost all the protein fractions had molecular weights ranging between 14 000 and 94 000 daltons. Preliminary comparisons between the larval and pupal blood protein spectra revealed some distributional and quantitative differences.
Keywords
Worker brood, protein fractions, densitometer scans
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