Distribution of protein
fractions in the blood of worker brood of Apis mellifera
Z. F. Glinski and J. Jarosz
Samples of blood were taken from larvae which had
straightened in their cells (10-11 days old), and from young pupae.
Polyacrylamide gel electrophoresis of the samples after they had been strongly
dissociated using a buffer, made possible the determination of detailed
distributional and quantitative characteristics of the blood proteins,
including the determination of molecular weights of particular protein
fractions or their subunits. Densitometer scans revealed 24 well-defined
fractions in larval blood and 27 in pupal blood. It was shown that after
dissociation almost all the protein fractions had molecular weights ranging
between 14 000 and 94 000 daltons. Preliminary comparisons between the larval
and pupal blood protein spectra revealed some distributional and quantitative differences.
Worker brood, protein fractions, densitometer scans