Diagnostic molecular markers discriminating Africanized honey bees from Greek and Cypriot honey bees (Apis mellifera, Hymenoptera: Apidae)

publication date: Dec 31, 2006
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Journal of Apicultural Research
Vol. 45 (4) pp. 197-202
DOI
10.3896/IBRA.1.45.4.05
Date
December 2006
 
Article Title
 
Diagnostic molecular markers discriminating Africanized honey bees from Greek and Cypriot honey bees (Apis mellifera, Hymenoptera: Apidae)
Author(s)
P C Harizanis,D I Nielsen and M Bouga
Abstract
Diagnostic patterns using PCR – RFLPs analysis of two mtDNA gene segments (16s rDNA and CO I) were shown to discriminate Africanized honey bees from Greek and Cypriot honey bees; high values of net nucleotide sequence divergence were observed among Africanized honey bees and the other populations studied. The tree obtained by UPGMA method ensured that the Africanized honey bee is discriminated from the honey bee populations from Greece and Cyprus. These results could be useful for the early detection of Africanized honey bees.
Keywords
Apis mellifera, honey bee, mtDNA, Africanized honey bee, Greece, Cyprus

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