Molecular cloning of two cDNAs from the head of the nurse honey bee (Apis mellifera L.) for coding related proteins of royal jelly

publication date: Sep 17, 2010
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Journal of Apicultural Research

Vol. 33 (2) pp. 105-111
Date
March 1994
Article Title

Molecular cloning of two cDNAs from the head of the nurse honey bee (Apis mellifera L.) for coding related proteins of royal jelly

Author(s)
J Klaudinv; J Hanes; J Kulifajova; S Albert; J Simuth
Abstract

Two cDNA clones designated pRJP57-1 and pRJP57-2 which code for related polypeptides of major royal jelly protein were identified from the AUni-ZAP XR library of nurse honey bee head cDNA. The nucleotide sequences of the cDNA inserts show a 70.2% identity. From the open reading frames of pRJP57-1 and pRJP57-2 the amino acid sequences for polypeptides of 467 and 464 amino acid residues were derived, showing an identity of 56.6%. No significant homology with hitherto known proteins was observed. Both proteins contain N-glycosylation sites, phosphorylation sites for three types of protein kinases and almost identical signal peptides. Northern analysis showed that total mRNA contains a high proportion of RJP57-1 and RJP57-2 mRNAs (8% and 2% respectively).

Keywords

worker honey bees, Apis mellifera, royal jelly, mRNA, rRNA, cDNA clones, signal peptides, proteins, polypeptides, phosphorylation, Northern blotting

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