Determination of carotenes from bee-collected pollen by high performance liquid chromatography
S. Muniategui, M. T. Sancho, J. Lopez, J. F. Huidobro And J. Simal
A fast method for the determination of the carotene fraction from commercially purchased bee-collected pollen by isocratic HPLC is described, using ultraviolet detection at 453 nm and direct injection of the sample into a silica column. The mobile phase was n-hexane:2-propanol:acetic acid 000:0.5:0.1), using flow 1 ml/min. The mean value for carotene was 46.7 mg β-carotene/100g lipids.
bee-collected pollen, carotenes, high performance liquid chromatography,