Determination of carotenes from bee-collected pollen by high performance liquid chromatography

publication date: Feb 18, 2011
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Journal of Apicultural Research
Vol. 29 (3) pp. 147-150
Date
September 1990
Article Title

Determination of carotenes from bee-collected pollen by high performance liquid chromatography  

Author(s)

S. Muniategui, M. T. Sancho, J. Lopez, J. F. Huidobro And J. Simal

Abstract

A fast method for the determination of the carotene fraction from commercially purchased bee-collected pollen by isocratic HPLC is described, using ultraviolet detection at 453 nm and direct injection of the sample into a silica column. The mobile phase was n-hexane:2-propanol:acetic acid 000:0.5:0.1), using flow 1 ml/min. The mean value for carotene was 46.7 mg β-carotene/100g lipids.

Keywords

bee-collected pollen, carotenes, high performance liquid chromatography,

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