Are globulin-like proteins present in larval blood of the honeybee Apis mellifera?

publication date: Jul 28, 2011
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Journal of Apicultural Research
Vol. 25 (2) pp. 71-77
Date
June 1986
Article Title

Are globulin-like proteins present in larval blood of the honeybee Apis mellifera?

Author(s)

Z. F. Glinski, J. Jarosz And A. Wernicki

Abstract

Proteins homologous to vertebrate immunoglobulins have been reported absent from the blood of several insect species. An investigation was made of the possible presence, in larval honeybee blood, of proteins migrating in the globulin region in immunoelectrophoresis. Blood of larvae 5 days old, either whole or fractionated with ethanol or ammonium sulphate, was analysed by polyacrylamide gel electrophoresis and immunoelectrophoresis using rabbit blood antiserum. One protein was found that appeared to be comparable to mammalian blood globulins; precipitation arcs associated with this protein migrated in the globulin region during immunoelectrophoresis of whole larval blood or of the globulin-like antigenic solution precipitated by ammonium sulphate. Other globulin-like or albumin-like proteins separated on acrylamide gel do not necessarily have the nature of mammalian globulins or albumins, since they migrated in both the albumin and globulin regions during immunoelectrophoresis.

Keywords

Blood proteins, larval blood, immunoelectrophoresis,

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