EFFECTS OF INSEMINATION ON THE INITIATION OF OVIPOSITION IN THE QUEEN HONEYBEE
OSMAN KAFTANOGLU AND YING-SHIN PENG
The number of days from emergence to the onset of oviposition in 7 groups of queens was as follows: naturally mated queens, 10'33 ± 0'68; free-flying queens treated with CO2, ll'OO ± 0'36; queens instrumentally inseminated by the Mackensen technique, 13'8 ± 1'94; virgin queen's treated with CO2, 14'00 ± 0'77; queens instrumentally inseminated, using the washing technique, 14'58 ± 0'53; queens injected with washing fluid, 15'82 ± 1'42; queens injec;ted with Kiev solution, 17'77 ± 1'24. The number of spermatozoa in the spermatheca of naturally mated queens, of queens inseminated by the Mackensen technique, and of queens inseminated by the washing technique, was 4'54 ± 0'17,3'83 ± 0'47, and 3'02 ± 0'52 millions, respectively. Naturally mated queens started laying eggs earlier than the instrumentally inseminated queens.