PRODUCTION OF HYDROGEN SULPHIDE BY BACILLUS LARVAE IN VITRO

publication date: Oct 22, 2012
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Journal of Apicultural Research
Vol. 21 (3) pp.161-164
DOI
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Date
September 1982
Article Title
PRODUCTION OF HYDROGEN SULPHIDE BY BACILLUS LARVAE IN VITRO
Author(s)
T. A. GOCHNAUER AND V. J. MARGETTS
Abstract
Laboratory cultures ofBacillus larvae, the causative agent of American foul brood disease, produced abundant hydrogen sulphide (HzS) if the medium contained added cysteine·HCI or sodium thiosulphate as a source of sulphur, but little or none from an unsupplemented medium or a medium supplemented with one of several other sulphur-containing compounds, including L-cystine, methionine, and glutathione. Cysteine' HCI (0'02%, w/v) added to the medium was rapidly metabolized by laboratory strains of B. larvae, and the cultures ceased to liberate HzS by 96 h. Freshly isolated strains, which sporulated well in culture, released very little or no HzS from the disulphide amino acid L-cystine during either growth or sporulation.
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