The distribution of male and female tissue in 25 cuticular structures was recordedfor 1555 adult honeybee gynandromorphs derived from 8 queens. Using the gynandromorph data, distances between structures on the blastoderm were calculated with a FORTRAN programme. The distances thus obtained were used in a BASIC programme to construct a detailed fate map showing the location of 24 structures. The distances between structures on the map fitted the gynandromorphic data well. The fate map was consistent with previously published maps for the honeybee, Drosophila and Habrobracon juglandis. Distances between structures on the blastoderm were not influenced by either the queen producing the gynandromorphs or the frequency of the gynandromorphs in a queen's progency. The median ocellus appeared to be a complex, interacting focus mapping at the site of the lateral ocellus with male bilaterally domineering.