Fluvalinate, a pyrethroid acaricide, is used to control Varroa jacobsoni in honey bee colonies. As a consequence, residues of the active ingredient accumulate in beeswax due to its highly lipophilic properties. Attempts have been made to reduce fluvalinate residues in beeswax by heating. The standard method of sterilizing beeswax by heating at 118°C under high pressure for one hour, used for manufacturing practices (Machova et al., 1992), resulted in a minor decrease of the initial concentration of fluvalinate residue in wax. Nine beeswax samples with fluvalinate residues were heated in this way, but the reduction of their residues was statistically insignificant (Vesely and Lenfcek, 1990). The method of microbial transformation of fluvalinate residues (Maloney et al., 1988) cannot decompose this substance when it is incorporated in beeswax.