Esterase inhibition during several immature worker stages of Apis mellifera following topical application of DDVP insecticide

publication date: May 6, 2010
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Journal of Apicultural Research
Vol. 35 (1) pp.37-43
Date
January 1996
 
Article Title

Esterase inhibition during several immature worker stages of Apis mellifera following topical application of DDVP insecticide

Author(s)

V L C Figueiredo; M M G Bitondi; Z L Paulino-Simões;

Abstract
DDVP (o,o-dimethyl-o-(2,2-dichlorovinyl) phosphate), an organophosphate insecticide and esterase inhibitor was applied to 5th-instar larvae and pre-pupae of Apis mellifera, to determine whether the esterase isoenzymes are essential for normal larvapupa transition. After treating larvae with 20 µg DDVP, we observed severe or total inhibition of activity of esterases Est-2, Est-2a, Est-3, Est-4 and Est-5. Only Est-1 activity was unaffected by this treatment. Nevertheless, all treated larvae transformed to prepupae and the prepupae-pupae transition and subsequent pupal development were followed by the recovery of Est-2, Est-3, Est-4 and Est-5 activities. However, the transition to pupae did not occur when prepupae were treated with DDVP. Electrophoretic analysis of prepupae after treatment showed that, except for Est-1, which was insensitive to DDVP, and Est-2a, which was not inhibited fully, the remaining esterases (Est-2, Est-3, Est-4 and Est-5) were inhibited permanently. A close correlation between recovery of isoenzyme activities and normal progress of development was observed. The possible causes of this developmental phase-specific sensitivity are discussed.
Keywords

Apis mellifera, worker honey bees, organic compounds, o,o-dimethyl-o-(2,2-dichlorovinyl) phosphate, DDVP, insecticide, esterases, isoenzymes, starch gel electrophoresis, pupae, larvae, topical application, brood development

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