Intercellular Connection between the lyrate organ and the growing oocyte in Varroa jacobsoni as revealed by Lucifer Yellow dye-coupling

publication date: Apr 28, 2010
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Journal of Apicultural Research
Vol. 36 (3-4) pp.145-149
Date
December 1997
 
Article Title

Intercellular Connection between the lyrate organ and the growing oocyte in Varroa jacobsoni as revealed by Lucifer Yellow dye-coupling

Author(s)

Frank Dittmann; Josefina Steiner

Abstract
Micro-injected Lucifer Yellow CH (LY) was used to study cytoplasmic continuity and transport between the lyrate organ and the developing oocyte in the ovary of the mite, Varroa jacobsoni, an ectoparasite of honey bees. When LY was micro-injected into the lyrate organ of young female mites in their phoretic phase, movement of the dye towards the late stage 2 oocyte did not occur. Further oocyte development was initiated by transfer of young female mites into brood cells of Apis mellifera shortly after operculation (‘reproductive phase' of mites). Uptake of haemolymph from an L5 (feeding phase) bee larva coincided with the migration of LY both from one lobe of the lyrate organ to the other as well as from the lyrate organ towards the oocyte. Since translocation of LY in vitro reflects the alimentation of oocytes (nutrimentary oogenesis), we highlight the temporal pattern of intercellular transport during oogenesis.
Keywords

reproduction, intercellular transport, lyrate organ, nutrimentary oogenesis, honey bee, Apis mellifera, Varroa jacobsoni

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