Procedures For The Culture Of Melissococcus Pluton From Diseased Brood And Bulk Honey Samples

publication date: Apr 21, 2010
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Journal of Apicultural Research
Vol. 37 (4) pp.293-294
Date
December 1998
 
Article Title

Procedures For The Culture Of Melissococcus Pluton From Diseased Brood And Bulk Honey Samples

Author(s)

Michael A Z Hornitzky; Lisa Smith

Abstract
European foulbrood (EFB), caused by Melissococcus pluton, was first confirmed in Australia in 1977 (Tham, 1978). The diagnosis of EFB in Australia is routinely achieved by the examination of carbol fuchsin stained smears prepared from diseased brood (Hornitzky, 1993). Larvae are considered to have EFB if smears contain lanceolate cocci typical of M. pluton. The presence of the common secondary invaders Enterococcus faecalis and/or Paenibacillus alvei is presumptive evidence of EFB (Bailey & Ball, 1991).
Keywords

European foulbrood, Melissococcus pluton, honey bee brood, culture techniques, honey

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