The effect of induced defecation in honey bee queens on the number and viability of spermatozoa in the spermatheca after instrumental insemination

publication date: Mar 28, 2010
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Journal of Apicultural Research
Vol. 49 (2) pp. 154-158
DOI
Date
April 2010
Article Title

The effect of induced defecation in honey bee queens on the number and viability of spermatozoa in the spermatheca after instrumental insemination

Author(s)

Krystyna Czekońska, Bożena Chuda-Mickiewicz and Paweł Chorbiński

Abstract

This study determined the number and viability of spermatozoa accumulated in the spermathecae of honey bee (Apis mellifera) queens which did or did not defecate prior to instrumental insemination. The study used 64 samples of semen from the spermathecae of 33 defecating queens and 31 queens that did not defecate. Spermatozoa counts were estimated by computer-assisted sperm analysis with an HTM IVOS analyser. The viability of spermatozoa was determined by fluorescence  staining with SYBR-14/propidium iodide (IP) and flow cytometry. Defecation by queens was found to have no effect on the number of  spermatozoa accumulated in the spermatheca, but it did affect their viability.

Keywords

Apis mellifera, queen honey bees, defecation, instrumental insemination, sperm

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