PCR detection of Kashmir bee virus in honey bee excreta

publication date: Dec 22, 2009
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Journal of Apicultural Research
Vol. 39 (3-4) pp. 103-106
DOI
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Date
December 2000
Article Title

PCR detection of Kashmir bee virus in honey bee excreta

Author(s)

A C F Hung

Abstract
Using direct RT-PCR, Kashmir bee virus (KBV) was detected in the faecal material of worker and queen honey bees (Apis mellifera). This occurrence implies that KBV could be transmitted horizontally in bee colonies without involving the suspected mite vector, Varroa jacobsoni. The molecular techniques described in this study can be used to certify KBV-free queens sold to beekeepers.
Keywords

Kashmir bee virus, Apis mellifera, detection, direct reverse transcription polymerase chain reaction, faeces, excretion, transmission

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