Development of a bioassay to test the orientation behaviour of the honey bee ectoparasite, Varroa jacobson

publication date: Dec 16, 2009
Send a summary of this page to someone via email.

Journal of Apicultural Research
Vol. 39 (1-2) pp. 47-54
DOI
--
Date
June 2000
 
Article Title

Development of a bioassay to test the orientation behaviour of the honey bee ectoparasite, Varroa jacobson

Author(s)

Monique N LeDoux; Stephen F Pernal; Heather A Higo; Mark L Winston

Abstract
A bioassay was developed to test various aspects of the orientation behaviour of the honey bee ectoparasite, Varroa jacobsoni. The bioassay arena consisted of a petri dish, 60 mm in diameter, in which live honey bee larvae and previously frozen adults were used as hosts. Bioassays were conducted in a dark incubator at 32°C for 60 min. Greater numbers of mites parasitized worker larvae than drone larvae, but this trend was not significant. Mites of various ages given a choice between nurse bees and fifth instar worker larvae preferred nurse bees at all ages of mites tested, excluding newly emerged mites. When given a choice between a nurse bee and a pollen forager, V. jacobson; did not show a preference, and similarly when given a choice between a nurse bee and an adult drone no preference was observed. Finally, when given a choice between a nurse bee extracted with hexane and a non-extracted nurse bee, V. jacobson; preferred the non-extracted host.
Keywords

worker honey bees, Apis mellifera, Varroa jacobsoni, mites, orientation behaviour, olfactory stimulation, bioassay, larvae, nurse bees, extraction

Download