This paper examines whether the effectiveness of insemination depends on induced defecation before insemination in honey bee (Apis mellifera) queens. The effectiveness of insemination was evaluated on the basis of mortality among queens, the number of queens commencing egg laying, and the length of the post-insemination interval to egg laying. All 64 queens from two breeding series underwent insemination; of these, 33 were induced to defecate immediately prior to the procedure, and 31 were the control group. Neither the number of dead queens nor the number of queens laying eggs differed between the studied groups. The queens that defecated before insemination started to lay eggs an average 1.3 days later than the control group.