A study on the genetic diversity of the Apis mellifera meda population in the south coast of the Caspian Sea using microsatellite markers
M. Royan,G. Rahimi, S. Esmaeilkhanian, S. Z. Mirhoseini and Z. Ansari
The genetic diversity of honey bees from the south strip area of the Caspian Sea (Guilan, Mazandaran and Golestan provinces in the north of Iran) was estimated using four microsatellite loci. For this purpose, DNA was extracted from the thoracic part of 100 worker honey bees from each province, according to the salting out method. Following amplification, products electrophoresed on 6% denaturing polyacrylamide gel. The results indicated that, the honey bees in the north of Iran had a low variability in terms of number of alleles per locus and heterozygosity. The results of this research confirm the previous morphological and biochemical indications of uniformity in the honey bee population of the south strip of the Caspian Sea despite a geographical spread of 1,000 km.