Toxic effects of methanolic and dichloromethane extracts of flowers and peduncles of Stryphnodendron adstringens (Leguminosae: Mimosoideae) on Apis mellifera and Scaptotrigona postica workers

publication date: Sep 30, 2006

Journal of Apicultural Research	Vol. 45 (3) pp. 112 -116
DOI	10.3896/IBRA.1.45.3.03
Date	September 2006
Article Title	Toxic effects of methanolic and dichloromethane extracts of flowers and peduncles of Stryphnodendron adstringens (Leguminosae: Mimosoideae) on Apis mellifera and Scaptotrigona postica workers
Author(s)	Tiago Favaro de Souza, Priscila Cintra, Osmar Malaspina, Odair Correa Bueno, João B. Fernandes, Sheila Susan M. da Silva Almeida
Abstract	Laboratory bioassays were conducted to evaluate toxicity of methanolic and dichloromethane extracts obtained from Stryphnodendron adstringens to Apis mellifera and Scaptotrigona postica workers. The extracts were incorporated into the diet of the bees for evaluation of mortality rates. The ingestion bioassays were made with three concentrations (0.002mg/g, 0.005mg/g and 0.01mg/g) for each bee species. The workers were kept in cages, with twenty workers per cage for each concentration tested. All bioassays had sixty workers in three cages that where maintained in a biological oxygen demand incubator with controlled temperature and humidity. The data obtained in the toxicity bioassays were analyzed statistically by Log Rank test and all methanolic and dichloromethane extracts showed significant (P<0.0001) toxic effects in all tested concentrations.
Keywords	botanical extracts, honey bees, “barbatimão,” toxicology
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