We report the presence of sacbrood virus (SBV) in colonies of the western honey bee (Apis mellifera L.) from the North Western Himalayan region of India. As in other regions of the world, the virus killed honey bee brood at the prepupal stage (10 days of age) on the second day after the sealing of brood. In 2003, SBV was detected at two locations in Himachal Pradesh. At the first location, Solan district, Nauni, it was detected in colonies during spring and summer (March to May) when it affected 0.39 % to 5.20 % of the brood. At the second location, Kangra district, Jachh (Himachal Pradesh), the disease was also detected during spring and summer (March to June) when it affected 0.23 % to 2.10 % of brood. The incidences of the disease were found to be significantly correlated with colony strength and brood rearing.
Apis mellifera, sacbrood virus, antiserum, electron microscopy, serology.