Quantitative determination of trans-10- Hydroxy-2-Decenoic Acid (10-HDA) in Brazilian royal jelly and commercial products containing royal jelly

publication date: Sep 30, 2007
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Journal of Apicultural Research
Vol. 46 (3) pp. 149-153
DOI
Date
September 2007
 
Article Title
 
Quantitative determination of trans-10- Hydroxy-2-Decenoic Acid (10-HDA) in Brazilian royal jelly and commercial products containing royal jelly
Author(s)
Celira Caparica-Santos and Maria Cristina Marcucci
Abstract
We report the analysis of Brazilian commercial bee products using high performance liquid chromatography, and a faster method, to detect trans-10-Hydroxy-2-Decenoic Acid or 10-Hydroxy-2-E-decenoic acid (10-HDA) in royal jelly. Determination was carried out using aqueous phosphoric acid 0.1% (pH 2.5)/methanol (50/50) as the mobile phase, and a LiChrospher® RP-18 column with detection at 215 nm. A good linearity was shown between the concentration of 10-HDA and peak area in the concentration range of 5.0-50.0 mg/L (r 0.99, n = 5). The within-day and between-day relative standard deviation (RSD) were1 % and 3 %, respectively.
Keywords
Brazilian royal jelly, 10-HDA, HPLC, bee products, health, GC-MS
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